Porcine Fibroblasts 100% Iberico

The NFC.SS1 cell line is a 100% Iberico Porcine Fibroblast cell line. Primary fibroblasts isolated from 100% Iberian (Ibérico) pigs sourced in Valladolid, Spain. This heritage breed is globally recognized for its distinctive lipid metabolism and tissue composition, making it a valuable model for applications in cultivated meat, extracellular matrix (ECM) production, and metabolic research.

Tissue was obtained via minimally invasive 3 mm dermal punch biopsy from neonatal animals (gluteal region), enabling the isolation of high-viability fibroblast populations while preserving tissue integrity and supporting strong proliferative capacity. The use of neonatal tissue contributes to enhanced growth performance and culture adaptability relative to adult-derived fibroblasts. Cells are adherent and exhibit characteristic fibroblast morphology with consistent proliferation across extended passaging. Cultures have been maintained beyond 40 passages to date with sustained growth and no observable senescence under standard conditions, suggesting extended lifespan potential compared to typical primary fibroblast lines.

Each cryopreserved vial contains approximately 1 × 10⁶ viable cells with high post-thaw recovery and attachment efficiency. All lots are tested negative for mycoplasma and screened for common adventitious agents. Full traceability is maintained from source tissue through cell banking and production.

These cells have been successfully used to generate structured cultivated pork prototypes, including pork loin formats produced on edible scaffolds. The resulting tissues demonstrate the cells’ capacity for scaffold integration, matrix deposition, and formation of cohesive, three-dimensional constructs. Representative prototype images are included to illustrate achievable tissue architectures and product formats.

These fibroblasts are well-suited for applications including cultivated meat R&D, and porcine cell biology, tissue engineering, and ECM production.

Each purchase includes a comprehensive documentation package (certificate of analysis, tech transfer protocol), along with access to technical support for process optimization, scale-up, and co-culture system development.